|
KMID : 0881720160310060439
|
|
Journal of Food Hygiene and Safety
2016 Volume.31 No. 6 p.439 ~ p.444
|
|
|
Comparative Evaluation of Real-Time PCR and Conventional Culture Method Using Two Selective Agars for the Detection of Cronobacter spp. in Powdered Infant Formula and Dried Pumpkin
|
|
Kim Hong-Seok
Shin Min-jung
Chon Jung-Whan
Lim Jong-Soo
Kim Young-Ji
Kim Dong-Hyeon
Chang Ho-Seok
Kim Hyun-Sook
Om Ae-Son
Oh Deog-Hwan
Song-Kwang-Young
Seo Kun-Ho
|
|
|
Abstract
|
|
|
|
In the present study, the performance of culture methods using two selective agars and real-time PCR were compared for selective isolation of Cronobacter in powdered infant formula and dried pumpkin. Two food samples were spiked with the pathogen and then preenriched in distilled water. A small portion of preenrichment (10 mL) was incubated in Enterobacteriaceae enrichment both, followed by inoculation onto Druggan-ForsytheIversen agar (DFI agar) and Cronobacter sakazakii chromogenic plating agar (R&F agar). The preenrichment and enrichment (1 mL each) was used in real-time PCR assay. In powdered infant formula (PIF), no statistical difference was observed between both culture methods and real-time PCR with preenrichemt (p > 0.05). However, the number of positives obtained by R&F agar and real-time PCR was much higher than that of culture method using DFI agar in dried pumpkin (p < 0.05). In particular, R&F agar yielded a significantly greater selectivity than DFI agar in dried pumpkin (p < 0.05). Real-time PCR and R&F agar, which are currently recommended by US FDA, could be used as an alternative detection tools for the isolation of Cronobacter in PIF and ingredient of child foods such as dried pumpkin that has high number of competing natural microflora.
|
|
|
KEYWORD
|
|
|
Cronobacter, DFI agar, R&F agar, real-time PCR, detection
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|